Material
   Arabidopsis
圖一

Arabidopsis plantlet

圖二

Flowers of Arabidopsis

圖三

Left panel shows the immature silique ,right panel shows the immature seeds inside the silique . Once the siliques turn into brown , it will split open and the seeds inside are mature.

圖四

Callus induced from root of Arabidopsis

Arabidopsis plantsecotype Columbia

The inflorescence of Arabidopsis

The mature silique of Arabidopsis

The collected Arabidopsis T1 seeds were selected with appropriate antibiotics.

 

   Tobacco

Tobacco T2 transformants

 

There is no significant difference in morphology between these pBI121 transformants and wild type plant.

 

Shoots regenerated from leaf disk of tobacco

The collected tobacco T1 seeds were selected with appropriate antibiotics.

 

 

  Rice

Rice transformation

After inoculated with Agrobacterium, embryogenic calli were selected with antibiotics.

 

Shoots regenerated from antibiotic resistant calli.

Removed the regenerated rice shoots to the root-induction media.

   Tomato

Removed the regenerated tomato shoots to the root-induction media.

 TRANSFORMATION

   Tobacco

tobacco leaf disks co-culture with Agrobacterium for 5min

 

wash inoculated leaf disks with sterilized ddH2O

dry these explants with sterilized paper towel

recover on TSM media without any antibiotics for 2 days

subculture to TSM with

appropriate antibiotics

 

 

 

 

incubate at 26 for 2-3 weeks, shooting from the edge of the explants

remove shoots to rooting media

  Arabidopsis

 

 

Pick up the optimal plants that have many immature flower clusters while not many fertilized siliques.

Remove all siliques and opened flowers.

 

 

 

 

flower buds before inoculation

 

 

plants were inverted into Agrobacterium suspension so that all above-ground tissues were submerged.

Vacuum was applied for 15 min, and then released the vacuum rapidly.

 

 

Remove plants to a plastic tray and cover with aluminium foil O/N, on the next day, remove the aluminium foil, and let the plants grow as normal

Rice

 SOWING Arabidopsis SEEDS